Posttranscriptional gene silencing is not compromised in the Arabidopsis CARPEL FACTORY (DICER-LIKE1) mutant, a homolog of dicer-1 from Drosophila


Autoria(s): Finnegan, E. Jean; Margis, Rogerio; Waterhouse, Peter M.
Data(s)

2003

Resumo

Posttranscriptional silencing (PTGS) in plants, nematodes, Drosophila, and perhaps all eukaryotes operates by sequence-specific degradation or translational inhibition of the target mRNA. These processes are mediated by duplexed RNA. In Drosophila and nematodes, double-stranded (ds)RNA or self-complementary RNA is processed into fragments of approximately 21 nt by Dicer-1 [1, 2]. These small interfering RNAs (siRNAs) serve as guides to target degradation of homologous single-stranded (ss)RNA [1, 3]. In some cases, the approximately 21 nt guide fragments derived from endogenous, imperfectly self-complementary RNAs cause translational inhibition of their target mRNAs, with which they have substantial, but not perfect sequence complementarity [4-6]. These small temporal RNAs (stRNAs) belong to a class of noncoding microRNAs (miRNAs), 20-24 nt in length, that are found in flies, plants, nematodes, and mammals [4, 6-12]. In nematodes, the Dicer-1 enzyme catalyzes the production of both siRNA and stRNA [2, 13-15]. Mutation of the Arabidopsis Dicer-1 homolog, CARPEL FACTORY (CAF), blocks miRNA production [1, 4, 16-18]. Here, we report that the same caf mutant does not block either PTGS or siRNA production induced by self-complementary hairpin RNA. This suggests either that this mutation only impairs miRNA formation or, more interestingly, that plants have two distinct dicer-like enzymes, one for miRNA and another for siRNAi production.

Identificador

http://eprints.qut.edu.au/65958/

Publicador

Cell Press

Relação

DOI:10.1016/S0960-9822(03)00010-1

Finnegan, E. Jean, Margis, Rogerio, & Waterhouse, Peter M. (2003) Posttranscriptional gene silencing is not compromised in the Arabidopsis CARPEL FACTORY (DICER-LIKE1) mutant, a homolog of dicer-1 from Drosophila. Current Biology, 13(3), pp. 236-240.

Direitos

Copyright 2003 Cell Press

Fonte

School of Earth, Environmental & Biological Sciences; Science & Engineering Faculty

Palavras-Chave #060400 GENETICS #060700 PLANT BIOLOGY #Animals #Arabidopsis #Arabidopsis Proteins #Cell Cycle Proteins #Drosophila melanogaster #Endoribonucleases #MicroRNAs #Multigene Family #Phylogeny #Ribonuclease III #RNA Interference #RNA #Small Interfering #Embryophyta #Eukaryota #Mammalia #Nematoda
Tipo

Journal Article