A novel bioreactor for ligament tissue engineering
Data(s) |
2008
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Resumo |
Bioreactors are defined as devices in which biological and/or biochemical processes develop under closely monitored and tightly controlled environmental and operating conditions (e.g. pH, temperature, mechanical conditions, nutrient supply and waste removal). In functional tissue engineering of musculoskeletal tissues, a bioreactor capable of controlling dynamic loading plays a determinant role. It has been shown that mechanical stretching promotes the expression of type I and III collagens, fibronectin, tenascin-C in cultured ligament fibroblasts (J.C.-H. Goh et al., Tissue Eng. 9 (2003), S31) and that human bone marrow mesenchymal stem cells (hBMMSC) – even in the absence of biochemical regulators – could be induced to differentiate into ligament-like fibroblast by the application of physiologically relevant cyclic strains (G. Vunjak-Novakovic et al., Ann. Rev. Biomed. Eng. 6 (2004), 131; H.A. Awad et al., Tissue Eng. 5 (1999), 267; R.G. Young et al., J. Orthop. Res. 16 (1998), 406). Different bioreactors are commercially available but they are too generic to be used for a given tissue, each tissue showing specific mechanical loading properties. In the case of ligament tissue engineering, the design of a bioreactor is still an open question. Our group proposes a bioreactor allowing cyclic traction–torsion on a scaffold seeded with stem cells. |
Identificador | |
Publicador |
IOS Press |
Relação |
DOI:10.3233/BME-2008-0538 Kahn, Cyril J.F., Vaquette, Cedryck, Rahouadj, Rachid, & Wang, Xiong (2008) A novel bioreactor for ligament tissue engineering. Bio-Medical Materials and Engineering, 18(4-5), pp. 283-287. |
Fonte |
Institute of Health and Biomedical Innovation; Science & Engineering Faculty |
Palavras-Chave | #090304 Medical Devices #Bioreactor #ligament #cell culture #stem cell #cell differentiation |
Tipo |
Journal Article |