Effectiveness of cysteine proteases on protein/pigment film removal
Data(s) |
2013
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Resumo |
Objective: Theaflavin (TF) from the black tea can react to human salivary proline-rich proteins (PRPs) to form stains on exposed dental surfaces. Here, we employed a model of protein/pigment film using TF and dephosphorylated bovine b-casein (Db-CN), which has an extended conformation, similar to that of salivary PRPs, on a sensor surface to assess the efficacy of cysteine proteases (CPs) including papain, stem bromelain, and ficin, on removing TF bound to Db-CN and the control TF readsorption on the residual substrate surfaces was also measured. Methods: The protein/pigment complex film was built by using a quartz crystal microbalance with dissipation (QCM-D). The efficacies of CPs were assessed by Boltzman equation model. The surface details were detected by grazing angle infrared spectroscopy spectra, atomic force microscopy images, and contact angles. Results: The efficacy order of CPs on hydrolyzing protein/pigment complex film is ficin > papain > bromelain. The results from grazing angle infrared spectroscopy spectra, atomic force microscopy images, and contact angles demonstrated that TF bound on the Db- CN was effectively removed by the CPs, and the amount of TF readsorption on both the residual film of the Db-CN/TF and the Db-CN was markedly decreased after hydrolysis. Conclusion: This study indicates the potential application of the CPs for tooth stain removal and suggests that these enzymes are worthy of further investigation for use in oral healthcare. |
Identificador | |
Publicador |
Elsevier |
Relação |
DOI:10.1016/j.archoralbio.2013.07.015 Yao, Jiang-Wu, Xiao, Yin, Zuo, Qi-Liang, Zhang, Yi, Tao, Tao, & Lin, Chang-Jian (2013) Effectiveness of cysteine proteases on protein/pigment film removal. Archives of Oral Biology, 58(11), pp. 1618-1626. |
Direitos |
Copyright 2013 Elsevier Ltd. |
Fonte |
School of Chemistry, Physics & Mechanical Engineering; Institute of Health and Biomedical Innovation; Science & Engineering Faculty |
Palavras-Chave | #060000 BIOLOGICAL SCIENCES #110500 DENTISTRY #Cysteine proteases #Dephosphorylated b-casein #Theaflavin #Hydrolysis #Film |
Tipo |
Journal Article |