Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry
Data(s) |
01/12/2008
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Resumo |
This paper was designed to study metabonomic characters of the hepatotoxicity induced by alcohol and the intervention effects of Yin Chen Hao Tang (YCHT), a classic traditional Chinese medicine formula for treatment of jaundice and liver disorders in China. Urinary samples from control, alcohol- and YCHT-treated rats were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-QTOF-MS) in positive ionization mode. The total ion chromatograms obtained from the control, alcohol- and YCHT-treated rats were easily distinguishable using a multivariate statistical analysis method such as the principal components analysis (PCA). The greatest difference in metabolic profiling was observed from alcohol-treated rats compared with the control and YCHT-treated rats. The positive ions m/z 664.3126 (9.00 min) was elevated in urine of alcohol-treated rats, whereas, ions m/z 155.3547 (10.96 min) and 708.2932 (9.01 min) were at a lower concentration compared with that in urine of control rats, however, these ions did not indicate a statistical difference between control rats and YCHT-treated rats. The ion m/z 664.3126 was found to correspond to ceramide (d18:1/25:0), providing further support for an involvement of the sphingomyelin signaling pathway in alcohol hepatotoxicity and the intervention effects of YCHT. |
Identificador | |
Publicador |
Elsevier |
Relação |
DOI:10.1016/j.jpba.2008.07.028 Wang, Xijun, Lv, Haitao, Sun, Hui, Liu, Lian, Yang, Bo, Sun, Wenjun, Wang, Ping, Zhou, Dixin, Zhao, Li, Dou, Shengshan, Zhang, Guangmei, & Cao, Hongxin (2008) Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 48(4), pp. 1161-1168. |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #Animals #Chromatography #High Pressure Liquid/*methods #Drug Toxicity #Drugs #Chinese Herbal/*metabolism #Ethanol/*toxicity #Hepatocytes/drug effects/pathology #Male #Medicine #Chinese Traditional/*methods #*Metabolome #Random Allocation #Rats #Wistar #Reproducibility of Results #Spectrometry #Mass #Electrospray Ionization/*methods #Urinalysis/methods #Urinary Tract/drug effects |
Tipo |
Journal Article |