Effect of a novel compound from Lycopodium obscurum L. on osteogenic activity of osteoblasts in vitro


Autoria(s): Wang, Chaoyuan; Wu, Ruiyun; Yang, Guangzhong; Blackwood, Keith A.; Friis, Thor; Hutmacher, Dietmar W.; Woodruff, Maria A.
Data(s)

01/01/2013

Resumo

We investigated the potential of an extract of Lycopodium obscurum L.; stigmastane-3-oxo-21-oic acid (SA), to enhance osteogensis of mouse osteoblastic MC3T3-E1 cells. SA at a concentration of 16 µM was found to have no significant effect upon the viability of the cells, thus concentrations of 8 µM and 16 µM of SA were used in all further experiments. Both concentrations of SA had an inhibitory affect upon alkaline phosphatase activity (ALP) after 8 days incubation, however, after 16 days activity was restored to control levels. However Alizarin red S staining showed increased levels of mineralization for both concentrations after 16 days culture. Real time PCR showed inhibition of genes Runx2 and Osterix genes responsible for the up-regulation of ALP. However early time point (8 days) up-regulation of bone matrix mineralization genes OPN and OCN, and late time point (16 days) up-regulation of both Jun-D and Fra-2 mRNA expression was significantly enhanced. These results suggest a potential me-chanism of SA in enhancing bone fracture healing is through the up-regulating bone matrix minera-lization.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/55475/

Publicador

SCIRP

Relação

http://eprints.qut.edu.au/55475/2/55475.pdf

DOI:10.4236/ns.2013.51014

Wang, Chaoyuan, Wu, Ruiyun, Yang, Guangzhong, Blackwood, Keith A., Friis, Thor, Hutmacher, Dietmar W., & Woodruff, Maria A. (2013) Effect of a novel compound from Lycopodium obscurum L. on osteogenic activity of osteoblasts in vitro. Natural Science, 5(1), pp. 84-92.

Direitos

Copyright 2013 SciRes.

Fonte

Institute of Health and Biomedical Innovation; Science & Engineering Faculty

Palavras-Chave #060100 BIOCHEMISTRY AND CELL BIOLOGY #090300 BIOMEDICAL ENGINEERING
Tipo

Journal Article