Effects of oxygen on zonal marker expression in human articular chondrocytes
Data(s) |
2012
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Resumo |
Articular cartilage is organized in depth zones with phenotypically distinct subpopulations of chondrocytes that are exposed to different oxygen tensions. Despite growing evidence of the critical role for oxygen in chondrogenesis, little is known about its effect on chondrocytes from different zones. This study evaluates zonal marker expression of human articular chondrocytes from different zones under various oxygen tensions. Chondrocytes isolated from full-thickness, superficial, and middle/deep cartilage from knee replacement surgeries were expanded and redifferentiated under hypoxic (5% O 2) or normoxic (20% O 2) conditions. Differentiation under hypoxia increased expression of hypoxia-inducible factors 1alpha and 2alpha and accumulation of extracellular matrix, particularly in middle/deep chondrocytes, and favored re-expression of proteoglycan 4 by superficial chondrocytes compared with middle/deep cells. Zone-dependent expression of clusterin varied with culture duration. These results demonstrate that zonal chondrocytes retain important phenotypic differences during in vitro cultivation, and that these characteristics can be improved by altering the oxygen environment. However, transcript levels for pleiotrophin, cartilage intermediate layer protein, and collagen type X were similar between zones, challenging their reliability as zonal markers for tissue-engineered cartilage from osteoarthritis patients. Key factors including oxygen tension and cell source should be considered to prescribe zone-specific properties to tissue-engineered cartilage. © 2012, Mary Ann Liebert, Inc. |
Formato |
application/pdf |
Identificador | |
Publicador |
Mary Ann Liebert, Inc. Publishers |
Relação |
http://eprints.qut.edu.au/50407/1/50407.pdf DOI:10.1089/ten.tea.2011.0088 Schrobback, K., Malda, J., Crawford, R. W., Upton, Z., Leavesley, D. I., & Klein, T. J. (2012) Effects of oxygen on zonal marker expression in human articular chondrocytes. Tissue Engineering Part A, 18(9-10), pp. 920-933. |
Direitos |
Copyright 2012 Mary Ann Liebert, Inc. Publishers This is a copy of an article published in the Tissue Engineering Part A (C) 2012 (copyright Mary Ann Liebert, Inc.); Tissue Engineering Part A is available online at: http://www.liebertonline.com. |
Fonte |
School of Chemistry, Physics & Mechanical Engineering; Faculty of Health; Institute of Health and Biomedical Innovation; Science & Engineering Faculty |
Palavras-Chave | #091300 MECHANICAL ENGINEERING |
Tipo |
Journal Article |