Effects of varied ionic calcium and phosphate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells in vitro
Data(s) |
2012
|
---|---|
Resumo |
Background and Objective: A number of bone filling materials containing calcium (Ca++) and phosphate (P) ions have been used in the repair of periodontal bone defects; however, the effect that local release of Ca++ and P ions have on biological reactions is not fully understood. In this study, we investigated the effects of various levels of Ca++ and P ions on the proliferation, osteogenic differentiation, and mineralization of human periodontal ligament cells (hPDLCs). Materials and Methods: hPDLCs were obtained using an explant culture method. Defined concentrations and ratios of ionic Ca++ to inorganic P were added to standard culture and osteogenic induction media. The ability of hPDLCs to proliferate in these growth media was assayed using the Cell Counting Kit-8 (CCK-8). Cell apoptosis was evaluated by FITC-Annexin V/PI double staining method. Osteogenic differentiation and mineralization were investigated by morphological observations, alkaline phosphatase (ALP) activity, and Alizarin red S/von Kossa staining. The mRNA expression of osteogenic related markers was analyzed using a reverse transcriptase polymerase chain reaction (RT-PCR). Results: Within the ranges of Ca++ and P ions concentrations tested, we observed that increased concentrations of Ca++ and P ions enhanced cell proliferation and formation of mineralized matrix nodules; whereas ALP activity was reduced. The RT-PCR results showed that elevated concentrations of Ca++ and P ions led to a general increase of Runx2 mRNA expression and decreased ALP mRNA expression, but gave no clear trend on OCN mRNA levels. Conclusion: The concentrations and ratios of Ca++ and P ions could significantly influence proliferation, differentiation, and mineralization of hPDLCs. Within the range of concentrations tested, we found that the combination of 9.0 mM Ca++ ions and 4.5 mM P ions were the optimum concentrations for proliferation, differentiation, and mineralization in hPDLCs. |
Formato |
application/pdf |
Identificador | |
Publicador |
John Wiley & Sons, Inc |
Relação |
http://eprints.qut.edu.au/46657/1/46657_submittedVersion.pdf DOI:10.1111/j.1600-0765.2011.01443.x An, Shaofeng, Ling , Junqi, Gao, Yan, & Xiao, Yin (2012) Effects of varied ionic calcium and phosphate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells in vitro. Journal of Periodontal Research, 47(3), pp. 374-382. |
Direitos |
Copyright 2011 John Wiley & Sons, Inc The definitive version is available at www3.interscience.wiley.com |
Fonte |
Faculty of Built Environment and Engineering; Institute of Health and Biomedical Innovation; School of Engineering Systems |
Palavras-Chave | #060100 BIOCHEMISTRY AND CELL BIOLOGY #110500 DENTISTRY #human periodontal ligament cells #calcium #phosphate #osteogenic differentiation #ceramics |
Tipo |
Journal Article |