Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines
Data(s) |
2002
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Resumo |
Mutations in exon 3 of the CTNNB1 gene encoding beta-catenin have been reported in colorectal cancer cell lines and tumours. Although one study reported mutations or deletions affecting beta-catenin in 20% of melanoma cell lines, subsequent reports detected a much lower frequency of aberrations in uncultured melanomas. To determine whether this difference in mutation frequency reflected an in vitro culturing artefact, exon 3 of CTNNB1 was screened in a panel of 62 melanoma cell lines. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect intragenic deletions affecting exon 3. One out of 62 (1.6%) cell lines was found to carry a mutation, indicating that aberration of the Wnt-1/wingless pathway through activation of beta-catenin is a rare event, even in melanoma cell lines. |
Formato |
application/pdf |
Identificador | |
Publicador |
Lippincott, Williams & Wilkins |
Relação |
http://eprints.qut.edu.au/45819/1/Pollock_Hayward_2002.pdf http://journals.lww.com/melanomaresearch/Abstract/2002/04000/Mutations_in_exon_3_of_the__beta__catenin_gene_are.13.aspx Pollock, Pamela M. & Hayward, Nicholas K. (2002) Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines. Melanoma Research, 12(2), pp. 183-186. |
Direitos |
Copyright 2002 Lippincott, Williams & Wilkins |
Fonte |
Cell & Molecular Biosciences; Faculty of Science and Technology; Institute of Health and Biomedical Innovation |
Palavras-Chave | #111201 Cancer Cell Biology #Cytoskeletal Proteins/ genetics #DNA Mutational Analysis #DNA #Neoplasm/genetics #Exons/genetics #Humans #Melanoma/ genetics/metabolism/pathology #Mutation/ genetics #Polymorphism #Single-Stranded Conformational #Reverse Transcriptase Polymerase Chain Reaction #Skin Neoplasms/ genetics/metabolism/pathology #Trans-Activators #Tumor Cells #Cultured #beta Catenin |
Tipo |
Journal Article |