Adult human articular chondrocytes in a microcarrier-based culture system : expansion and redifferentiation
Data(s) |
18/10/2010
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Resumo |
xpanding human chondrocytes in vitro while maintaining their ability to form cartilage remains a key challenge in cartilage tissue engineering. One promising approach to address this is to use microcarriers as substrates for chondrocyte expansion. While microcarriers have shown beneficial effects for expansion of animal and ectopic human chondrocytes, their utility has not been determined for freshly isolated adult human articular chondrocytes. Thus, we investigated the proliferation and subsequent chondrogenic differentiation of these clinically relevant cells on porous gelatin microcarriers and compared them to those expanded using traditional monolayers. Chondrocytes attached to microcarriers within 2 days and remained viable over 4 weeks of culture in spinner flasks. Cells on microcarriers exhibited a spread morphology and initially proliferated faster than cells in monolayer culture, however, with prolonged expansion they were less proliferative. Cells expanded for 1 month and enzymatically released from microcarriers formed cartilaginous tissue in micromass pellet cultures, which was similar to tissue formed by monolayer-expanded cells. Cells left attached to microcarriers did not exhibit chondrogenic capacity. Culture conditions, such as microcarrier material, oxygen tension, and mechanical stimulation require further investigation to facilitate the efficient expansion of clinically relevant human articular chondrocytes that maintain chondrogenic potential for cartilage regeneration applications. |
Formato |
application/pdf |
Identificador | |
Publicador |
Orthopedic Research Society |
Relação |
http://eprints.qut.edu.au/39222/1/41101.pdf DOI:10.1002/jor.21264 Schrobback, Karsten, Klein, Travis J., Scheutz, Micheal, Upton, Zee, Leavesley, David I., & Malda, Jos (2010) Adult human articular chondrocytes in a microcarrier-based culture system : expansion and redifferentiation. Journal of Orthopedic Research, 29(4), pp. 539-546. |
Direitos |
Copyright 2010 Orthopaedic Research Society |
Fonte |
Cell & Molecular Biosciences; Faculty of Built Environment and Engineering; Faculty of Science and Technology; Institute of Health and Biomedical Innovation; School of Engineering Systems; Science Research Centre |
Palavras-Chave | #060106 Cellular Interactions (incl. Adhesion Matrix Cell Wall) #060199 Biochemistry and Cell Biology not elsewhere classified #110314 Orthopaedics #111602 Human Biophysics #cartilage #tissue engineering #microcarrier #chondrocyte #dedifferentiation |
Tipo |
Journal Article |