Simultaneous determination of three synthetic glucocorticoids by differential pulse stripping voltammetry with the aid of chemometrics


Autoria(s): Ni, Yongnian; Li, Shuzhen; Kokot, Serge
Data(s)

01/01/2008

Resumo

A novel voltammetric method for simultaneous determination of the glucocorticoid residues prednisone, prednisolone, and dexamethasone was developed. All three compounds were reduced at a mercury electrode in a Britton-Robinson buffer (pH 3.78), and well-defined voltammetric waves were observed. However, the voltammograms of these three compounds overlapped seriously and showed nonlinear character, and thus, it was difficult to analyze the compounds individually in their mixtures. In this work, two chemometrics methods, principal component regression (PCR) and partial least squares (PLS), were applied to resolve the overlapped voltammograms, and the calibration models were established for simultaneous determination of these compounds. Under the optimum experimental conditions, the limits of detection (LOD) were 5.6, 8.3, and 16.8 µg l-1 for prednisone, prednisolone, and dexamethasone, respectively. The proposed method was also applied for the determination of these glucocorticoid residues in the rabbit plasma and human urine samples with satisfactory results.

Identificador

http://eprints.qut.edu.au/29038/

Publicador

Taylor & Francis

Relação

DOI:10.1080/00032710802208708

Ni, Yongnian, Li, Shuzhen, & Kokot, Serge (2008) Simultaneous determination of three synthetic glucocorticoids by differential pulse stripping voltammetry with the aid of chemometrics. Analytical Letters, 41(11), pp. 2058-2076.

Fonte

Faculty of Science and Technology; School of Physical & Chemical Sciences

Palavras-Chave #Chemometrics #Differential Pulse Stripping Voltammetry #Glucocorticoid Residues #Rabbit Plasma #Urine
Tipo

Journal Article