Antimicrobial susceptibility patterns, emm type distribution and genetic diversity of Streptococcus pyogenes recovered in Brazil


Autoria(s): Arêas, Glauber P.; Schuab, Rôde B.B.; Neves, Felipe P.G.; Barros, Rosana R.
Cobertura

Origin of publication: Brazil

Data(s)

23/01/2015

Resumo

Streptococcus pyogenes is responsible for a variety of infectious diseases and immunological complications. In this study, 91 isolates of S. pyogenes recovered from oropharynx secretions were submitted to antimicrobial susceptibility testing, emm typing and pulsed-field gel electrophoresis (PFGE) analysis. All isolates were susceptible to ceftriaxone, levofloxacin, penicillin G and vancomycin. Resistance to erythromycin and clindamycin was 15.4%, which is higher than previous reports from this area, while 20.9% of the isolates were not susceptible to tetracycline. The macrolide resistance phenotypes were cMLSB (10) and iMLSB (4). The ermB gene was predominant, followed by the ermA gene. Thirty-two emm types and subtypes were found, but five (emm1, emm4, emm12, emm22, emm81) were detected in 48% of the isolates. Three new emm subtypes were identified (emm1.74, emm58.14, emm76.7). There was a strong association between emm type and PFGE clustering. A variety of PFGE profiles as well as emm types were found among tetracycline and erythromycin-resistant isolates, demonstrating that antimicrobial resistant strains do not result from the expansion of one or a few clones. This study provides epidemiological data that contribute to the development of suitable strategies for the prevention and treatment of such infections in a poorly studied area.

Formato

html

Identificador

http://www.bioline.org.br/abstract?id=oc14137

Idioma(s)

en

Publicador

Fundação Oswaldo Cruz, Fiocruz

Relação

http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc

Direitos

Copyright 2014 - Memórias do Instituto Oswaldo Cruz

Fonte

Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 109 Num 7

Palavras-Chave #Streptococcus pyogenes; antimicrobial resistance; epidemiological typing; genetic diversity
Tipo

AA